bet亚洲官网手机版-bet1 365 vip官方网站

产品资料
产品[

美国Sox1-GFP报告基因小鼠胚胎干细胞

]资料
如果您对该产品感兴趣的话,可以 sendmsg
产品名称: 美国Sox1-GFP报告基因小鼠胚胎干细胞
产品型号: PCEMM01
产品厂商: 美国PrimCells
产品文档: 无相关文档


简单先容

美国Sox1-GFP报告基因小鼠胚胎干细胞 以美国Sox1-GFP报告基因小鼠胚胎干细胞等为代表的美国PrimCells干细胞:PrimCells提供多种报告基因敲进的小鼠胚胎干细胞,该类细胞皆在原始基因座上敲入荧光报告基因,从而使荧光蛋白的表达能真实反映原基因座附近的表达调控状况。该类细胞作为研究胚胎干细胞自更新和定向分化的有力工具,受到多家杰出研究所研究人员的青睐。


美国Sox1-GFP报告基因小鼠胚胎干细胞 的详细先容
美国Sox1-GFP报告基因小鼠胚胎干细胞

美国PrimCells干细胞:PrimCells提供多种报告基因敲进的小鼠胚胎干细胞,该类细胞皆在原始基因座上敲入荧光报告基因,从而使荧光蛋白的表达能真实反映原基因座附近的表达调控状况。该类细胞作为研究胚胎干细胞自更新和定向分化的有力工具,受到多家杰出研究所研究人员的青睐。


Species: Mus Musculus Catalog Number: PCEMM01
Cell Number: ~2x106 Storage/Shipping: Dry Ice


Introduction:

Sox1 is a transcriptional factor from SOX protein family. It is one of the earliest and most specific markers for neural progenitors and expressed throughout the central neural system during early embryo development1, 2. PrimCells is proud to isolate and provide to the research community the culture of Sox1-EGFP ESCs from transgenic knock-in mice. These cells were cultured in standard ESCs medium on gelatin coated plates and be used as a powerful tool to examine the process by which mESCs acquire neural identity.

ES cell culture and monolayer neural differentiation procudures

Sox1-GFP mESCs should be maintained in the stem cell culture medium to keep the pluripotent state (Figure 1).

For monoculture neural differentiation, dissociate and plate the undifferentiated Sox1-GFP mESCs  on to 0.1% gelatin-coated tissue culture plastic at a density of 1-3x104/cm2 in serum-free N2B27 medium. Renew N2B27 medium every 2 days. GFP positive cells can be observed on Day 5 (Figure 2).


Figure 1 | Sox1-GFP mESCs express typical ES cell markers


Figure 2 |  Monoculture neuronal differentiation of Sox1-GFP mESCs on Day 5

Growth Medium

Embryonic stem cell medium

DMEM:   415ml

FBS: 75ml (HYCLONE LABORATORIES INC, Cat# SH30070.03EH)

L-Glutamine: 5ml

MEM NEAA: 5ml

1-Thioglycerol: 4.3ul

LIF: 50ul LIF: 50ul (10ng/ml final concentration)  (PrimCells, Cat# PFHSA01)

Total Volume: 500ml

N2B27 medium for neural differentiation

N2B27 is a 1:1 mixture of DMEM/F12 (Gibco, Paisley, UK) supplemented with modified N2 (25 ?g/ml insulin, 100 ?g/ml apo-transferrin, 6 ng/ml progesterone, 16 ?g/ml putrescine, 30 nM sodium selenite and 50 ?g/ml bovine serum albumin fraction V (Gibco)) and Neurobasal medium supplemented with B27 (both from Gibco).

Alternatively, Ndiff227 (StemCells INC, Cat# SCS-SF-NB-02) is ready-to-use medium for the neural differentiation of mESCs  in adherent monoculture conditions as described in Ying QL et al (2003)2.

References

1.   Aubert, J. et al. Screening for mammalian neural genes via fluorescence-activated cell sorter purification of neural precursors from Sox1-gfp knock-in mice. Proc Natl Acad Sci U S A 100 Suppl 1, 11836-11841 (2003).

2.   Ying, Q.L., Stavridis, M., Griffiths, D., Li, M. & Smith, A. Conversion of embryonic stem cells into neuroectodermal precursors in adherent monoculture. Nat Biotechnol 21, 183-186 (2003).

美国PrimCells品牌先容
PrimCells是位于美国加州圣迭戈的一家专业致力于细胞产品研发和解决方案提供的业内技术领导企业。
企业汇集了**的生物医药技术专家,独立开发了多项原代细胞和干细胞相关的专有技术。 企业以“细胞研究的*优选择”为宗旨,自主研发超过70种**产品,填补了多个特种细胞市场的空白。例如其诱导性表达细胞库和高效3D皮肤培养系统,皆为该类产品目前市场上少数的规模化供应商。此外,企业以高质量的数据与快速稳定的项目完成时间,长期为多家知名制药企业提供解决方案,获得用户广泛赞誉。   
PrimCells产品线:
  • 报告基因干细胞        2. 原代细胞(含报告基因原代细胞)               3. 诱导性稳定细胞株
4. 人源重组蛋白               5.   **型培养试剂      
PrimCells 解决方案
1.诱导性稳定细胞株开发 2. 原代细胞开发 3. **体外毒理和效能测试 4. 3D-皮肤培养
PrimCells合作伙伴及客户:
A.生物制药企业:辉瑞(Pfizer), 诺华(Novartis), 礼来(Eli2  Lilly),. Amgen, EMD Millipore, Regeneron等企业的毒理和医药研发部门
B.科研机构:冷泉港实验室,哈佛医学院, 麻省理工(MIT),, UCSF, NYU, Salk, UIUC, USC等
产品留言
标题
联系人
联系电话
内容
验证码
点击换一张
注:1.可以使用快捷键Alt+S或Ctrl+Enter发送信息!
2.如有必要,请您留下您的详细联系方式!

联系大家
产品搜索

细胞系,ATCC细胞,ATCC菌种,细胞系目录,ATCC,鼠伤寒沙门氏菌,atcc细胞库,人淋巴瘤细胞,人食管癌细胞,ATCC 19404,ATCC 25922,ATCC 19146,微生物菌种,细胞株,标准菌株,ATCC中国,atcc上海代理,CMCC 菌株,ATCC菌株,细胞目录,质控菌株,肿瘤细胞株,乳腺癌细胞系,人肝癌细胞系

产品目录

Copyright? 2003-2021  上海复祥生物科技有限企业版权所有            电话:021-35080510
传真:021-35080520    地址:上海市虹口区赤峰路630号南雁大厦1901室    邮编:   

  

bet亚洲官网手机版|bet1 365 vip官方网站

XML 地图 | Sitemap 地图