bet亚洲官网手机版-bet1 365 vip官方网站

产品资料
产品[

CRL2254AML12小鼠肝细胞

]资料
如果您对该产品感兴趣的话,可以 sendmsg
产品名称: CRL2254AML12小鼠肝细胞
产品型号: CRL-2254 AML-12
产品厂商: 美国标准生物品收藏中心(ATCC)
产品文档: 无相关文档


简单先容

CRL-2254 AML-12 小鼠肝细胞,ATCC 细胞|细胞系|细胞株|肿瘤细胞|细胞;细胞库管理规范,提供的细胞株背景清楚,提供参考文献和*优培养条件!


CRL2254AML12小鼠肝细胞 的详细先容
CRL-2254 AML-12 小鼠肝细胞
Organism Mus musculus, mouse
Tissue liver
Cell Type hepatocyte
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Disease normal
Age 3 months
Gender male
Strain CD-1           CRL-2254 AML-12 小鼠肝细胞
Applications
This cell line is a suitable transfection host.
Storage Conditions liquid nitrogen vapor phase
Derivation
The AML12 (alpha mouse liver 12) cell line was established from hepatocytes from a mouse (CD1 strain, line MT42) transgenic for human TGF alpha.
Clinical Data
male
Genes Expressed
albumin; human transforming growth factor alpha (TGF alpha); mouse TGF alpha. 
Tumorigenic No
Effects
No, the cells do not form colonies or grow in soft agar.
No, the cells were not tumorigenic in immunosuppressed mice
Comments
The AML12 (alpha mouse liver 12) cell line was established from hepatocytes from a mouse (CD1 strain, line MT42) transgenic for human TGF alpha.
By electron microscopy, these cells exhibit typical hepatocyte features such as peroxisomes and bile canalicular like structure.
AML12 cells retain the capacity to express high levels of mRNA for serum (albumin, alpha 1 antitrypsin and transferrin) and gap junction (connexins 26 and 32) proteins, and contain solely isoenzyme 5 of lactate dehydrogenase.
The cells express high levels of human TGF alpha and lower levels of mouse TGF alpha.
Expression of liver specific proteins decreases with time in culture, but is reactivated by growing the cells in serum free medium.        CRL-2254 AML-12 小鼠肝细胞
Complete Growth Medium A 1:1 mixture of Dulbeccos modified Eagles medium and Hams F12 medium with 0.005 mg/ml insulin, 0.005 mg/ml transferrin, 5 ng/ml selenium, and 40 ng/ml dexamethasone, 90%; fetal bovine serum, 10% 
 
Subculturing Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. 

  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.
CRL-2254 AML-12 小鼠肝细胞
Subcultivation Ratio: 1:4 to 1:6
Medium Renewal: 2 to 3 times a week.
Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994.

 
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37°C
产品留言
标题
联系人
联系电话
内容
验证码
点击换一张
注:1.可以使用快捷键Alt+S或Ctrl+Enter发送信息!
2.如有必要,请您留下您的详细联系方式!

联系大家
产品搜索

细胞系,ATCC细胞,ATCC菌种,细胞系目录,ATCC,鼠伤寒沙门氏菌,atcc细胞库,人淋巴瘤细胞,人食管癌细胞,ATCC 19404,ATCC 25922,ATCC 19146,微生物菌种,细胞株,标准菌株,ATCC中国,atcc上海代理,CMCC 菌株,ATCC菌株,细胞目录,质控菌株,肿瘤细胞株,乳腺癌细胞系,人肝癌细胞系

产品目录

Copyright? 2003-2021  上海复祥生物科技有限企业版权所有            电话:021-35080510
传真:021-35080520    地址:上海市虹口区赤峰路630号南雁大厦1901室    邮编:   

  

bet亚洲官网手机版|bet1 365 vip官方网站

XML 地图 | Sitemap 地图