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产品名称: 美国National Diagnostics企业Autofluor放射自显影增强剂
产品型号: SG LS-315
产品厂商: 进口
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美国National Diagnostics企业Autofluor放射自显影增强剂美国 National Diagnostics企业原装产品


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美国National Diagnostics企业Autofluor放射自显影增强剂

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National Diagnostics

美国National Diagnostics企业Autofluor放射自显影增强剂

 

  • High resolution autoradiographic image intensifier
  • Rapid enhancement of low energy beta-emitters
  • For polyacrylamide gels, paper chromatography, and TLC plates
  • Water based, odorless, contains no DMSO

Catalog Number:SG LS-315

1. 高分辨率的放射自显影增强剂

2. 迅速增强 3 H,  14 C,  35 S等低能量的β放射

3. 适用于聚丙烯酰胺凝胶,纸层析和薄层层析

4. 水性无味液体,不含DMSO

Autofluor放射自显影增强剂**将水溶性闪烁荧光剂发展应用于放射自显影增强方面。它可以迅速渗入丙烯酰胺凝胶并将标记物传递给荧光剂的能量放大。它不含有DMSO也不含有任何芳香族的溶剂,避免了使用这些材料所带来的危害。同时,使用非水溶性增强剂造成的条带失真变形也不会存在。Autofluor放射自显影增强剂是目前增强检测β放射的所有方法中*简单省时的一种。在八种独立的聚丙烯酰胺凝胶中  35 S标记蛋白的放射自显影比较实验  Autofluor是*有效的。使用Autofluor后半饱和x光片的dpm/mm 2 值只需要单独显影的1/8。Autofluor应避免阳光直射,室温下可以至少保存一年。避免冷冻,温度低于20摄氏度时水溶性磷光剂可能会沉淀析出,加热到30摄氏度可以使得磷光剂复溶。

Autofluor represents the first water soluble scintillation phosphor to be developed and applied directly for use as an autoradiographic image intensifier. Autofluor rapidly penetrates acrylamide gel systems and maximizes energy transfer from labeled compound to phosphor. Autofluor contains no dimethylsulfoxide or acidic aromatic solvents. Therefore, the hazards of use related to these materials are eliminated. The band distortion that is associated with using nonaqueous enhancers is also eliminated.

In the figure to the right, the gel on the left (7%, 1mm) was dehydrated in DMSO (dimethylsulfoxide) for one hour, then impregnated in PPO-DMSO for one hour and precipitated and dried. The gel on the right was impregnated with Autofluor for one hour and dried. Both gels were exposed for 24 hours at -76°C on Kodak XR-5 X-OMat film. The single tritiated band contains 5000 dpm. Note the higher degree of resolution and band discrimination with Autofluor vs PPO-DMSO.

The Autofluor procedure is the shortest and easiest procedure yet developed for enhancement and visualization of beta-emitters. In an independent test comparing eight different fluorographic methods for the detection of 35S-labeled proteins in polyacrylamide gels, Autofluor was the most effective. With Autofluor, the dpm/mm2 required to half-saturate the x-ray film was 1/8 that required by autoradiography alone.

Storage: Autofluor has a shelf life of at least one year stored at room temperature, out of direct sunlight. Keep from freezing. At temperatures less than 20°C precipitation of water soluble phosphors may occur. Warming to approximately 30°C will redissolve the phosphors. 
 

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