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CCL-9.1 NCTC 1469 小鼠正常肝细胞

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产品名称: CCL-9.1 NCTC 1469 小鼠正常肝细胞
产品型号: CCL-9.1
产品厂商: 美国标准生物品收藏中心(ATCC)
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CCL-9.1 NCTC 1469 小鼠正常肝细胞 的详细先容
CCL-9.1 NCTC 1469 小鼠正常肝细胞
ATCC® Number: CCL-9.1™    Price: $329.00
Designations: NCTC clone 1469 [derivative of NCTC 721]
Depositors:  VJ Evans
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Mus musculus (mouse)
Morphology: epithelial

Source: Organ: liver
Strain: C3H/An
Disease: normal
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Isolation: Isolation date: April, 1952
Virus Susceptibility: Vesicular stomatitis virus
Human poliovirus 1
Antigen Expression: H-2k
Cytogenetic Analysis: modal number = 41; range = 38 to 86.
Two marker chromosomes present: One metacentric with one pair of arms having achromatic gaps near centromere; and an acrocentric with satellites.
Age: newborn
Gender: male
Comments: Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: horse serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol: Subcultures are prepared by scraping. Remove old medium, add fresh medium and dislodge the cells from the floor of the flask. Aspirate cells and dispense the suspension into new flasks.
A standard trypsinizaton may be used if desired.
The culture medium in heavy cultures may be turbid in appearance and and give the gross impression of bacterial contamination.
It is characteristic of this line to shed viable cells into the medium, thus rendering the medium turbid. The shed cells are viable and may be used to initiate new cultures.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: 3 times per week
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2040
References: 22167: Hobbs GL, et al. Establishment of a clone of mouse liver cells from a single isolated cell. J. Natl. Cancer Inst. 18: 701-707, 1957. PubMed: 13502690
26070: Evans VJ, et al. The growth in vitro of massive cultures of liver cells. J. Natl. Cancer Inst. 12: 1245-1265, 1952. PubMed: 14939026
26074: . . J. Natl. Cancer Inst. 23: 823, 1959.
26076: . . J. Natl. Cancer Inst. 27: 29, 1961.
26078: Evans VJ, et al. Studies on culture lines derived from mouse liver parenchymatous cells grown in long-term tissue culture. Cancer Res. 12: 261-266, 1958. PubMed: 13523589
26079: . . Fed. Proc. 17: 967, 1958.
26081: Westfall BB. Characterization of cells in tissue culture. Natl. Cancer Inst. Monogr. 7: 147-158, 1962. PubMed: 14006338


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