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产品名称: 小鼠血液细胞
产品型号: TIB-68
产品厂商: 美国标准生物品收藏中心(ATCC)
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TIB-68 WEHI-3 小鼠血液细胞,原代细胞、细胞系。细胞库管理规范,提供的细胞株背景清楚,提供参考文献和*优培养条件,


小鼠血液细胞 的详细先容
TIB-68 WEHI-3 小鼠血液细胞
ATCC® Number: TIB-68?    Price: $303.00
Designations: WEHI-3
Depositors:  M Cohn
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension (some adherent cells)
Organism: Mus musculus (mouse)
Morphology:
Source: Organ: peripheral blood
Strain: BALB/c
Disease: leukemia
Cellular Products: lysozyme; granulocyte colony stimulating activity (CSA); interleukin-3 (interleukin 3, IL-3)
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
 
Receptors: complement (C3)
Comments: The growth of WEHI-3 is inhibited by 4 ng/ml LPS and blocked by higher concentrations.
Dextran sulfate at 30 to 40 mcg/ml also inhibits growth.
Latex beads are phagocytized but are not toxic.
Zymosan and BCG are phagocytized and block growth.
The cells exhibit only weak effector activity in antibody dependent cell mediated cytotoxicity.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: Iscoves modified Dulbeccos medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and supplemented with 0.05 mM 2-mercaptoethanol, 90%; fetal bovine serum, 10%
Temperature: 37.0°C
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 X 10 exp5 viable cells/ml.
Maintain cell density between 2 X 10 exp5 and 2 X 10 exp6 viable cells/ml.
Adherent cells may be harvested by scraping.
Preservation: culture medium 95%; DMSO, 5%
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2005
recommended serum:ATCC 30-2020
References: 1080: Ralph P, et al. Lysozyme synthesis by established human and murine histiocytic lymphoma cell lines. J. Exp. Med. 143: 1528-1533, 1976. PubMed: 1083890
1135: Ralph P, Nakoinz I. Antibody-dependent killing of erythrocyte and tumor targets by macrophage-related cell lines: enhancement by PPD and LPS. J. Immunol. 119: 950-954, 1977. PubMed: 894031
1136: Ralph P, Nakoinz I. Direct toxic effects of immunopotentiators on monocytic myelomonocytic, and histiocytic or macrophage tumor cells in culture. Cancer Res. 37: 546-550, 1977. PubMed: 318922
23431: McKean DJ, et al. Major histocompatibility complex-restricted antigen presentation to antigen-reactive T cells by B lymphocyte tumor cells. J. Exp. Med. 154: 1419-1431, 1981. PubMed: 6170720
33035: Boring L, et al. Molecular cloning and functional expression of murine JE (monocyte chemoattractant protein 1) and murine macrophage inflammatory protein 1alpha receptors. J. Biol. Chem. 271: 7551-7558, 1996. PubMed: 8631787
 
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