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CRL-1927 SV40 MES 13 小鼠肾小球系膜细胞

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产品名称: CRL-1927 SV40 MES 13 小鼠肾小球系膜细胞
产品型号: CRL-1927
产品厂商: 美国标准生物品收藏中心(ATCC)
产品文档: 无相关文档


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CRL-1927 SV40 MES 13 小鼠肾小球系膜细胞 的详细先容
CRL-1927 SV40 MES 13 小鼠肾小球系膜细胞
ATCC® Number: CRL-1927™    Price: $355.00
Designations: SV40 MES 13
Depositors:  LJ Striker
Biosafety Level: 2 [Cells contain polyomavirus DNA sequences ]
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Mus musculus (mouse)
Morphology: myofibroblast-like

Source: Organ: kidney
Tissue: glomerulus
Cell Type: mesangial cell;
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Age: 7 to 10 weeks
Comments: The cells display prominent cytoskeletal staining for actin, and have abundant parallel fibrils in the cytoplasm. They are reported to contract in the presence of 1 X 10(-6) M angiotensin II. The cells form colonies in soft agar, and are positive for SV40 large T antigen.
Propagation: ATCC complete growth medium: The base medium for this cell line is 3:1 mixture of ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002 and Hams F12 medium with 14 mM HEPES.To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 5%.
Temperature: 37.0°C
Subculturing: Protocol:
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.

Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:10 is recommended
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Doubling Time: 26 hrs
Related Products: recommended serum:ATCC 30-2020
References: 22754: MacKay K, et al. Glomerular epithelial, mesangial, and endothelial cell lines from transgenic mice. Kidney Int. 33: 677-684, 1988. PubMed: 2835539
51029: Robey RB, et al. Regulation of mesangial cell hexokinase activity by PKC and the classic MAPK pathway. Am. J. Physiol. 277: F742-F749, 1999. PubMed: 10564237
51030: Robey RB, et al. Thrombin is a novel regulator of hexokinase activity in mesangial cells. Kidney Int. 57: 2308-2318, 2000. PubMed: 10844601
61211: Robey RB, et al. Regulation of mesangial cell hexokinase activity and expression by heparin-binding epidermal growth factor-like growth factor: epidermal growth factors and phorbol esters increase glucose metabolism via a common mechanism involving classic mitogen-activated protein kinase pathway activation and induction of hexokinase II expression. J. Biol. Chem. 277: 14370-14378, 2002. PubMed: 11782486
61224: Maile S, et al. The morphology of mesangial cells cultured at high density and in collagen gels. Histol. Histopathol. 15: 403-414, 2000. PubMed: 10809358
61225: Coy PE, et al. LPA is a novel lipid regulator of mesangial cell hexokinase activity and HKII isoform expression. Am. J. Physiol. Renal Physiol. 283: F271-F279, 2002. PubMed: 12110510
90657: Taneja N, et al. Proinflammatory interleukin-1 cytokines increase mesangial cell hexokinase activity and hexokinase II isoform abundance. Am. J. Physiol. Cell Physiol. 287: C548-C557, 2004. PubMed: 15070811


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