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HB-12317 NG108-15 小鼠神经细胞瘤与大鼠神经胶质瘤之融合细胞

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产品名称: HB-12317 NG108-15 小鼠神经细胞瘤与大鼠神经胶质瘤之融合细胞
产品型号: HB-12317
产品厂商: 美国标准生物品收藏中心(ATCC)
产品文档: 无相关文档


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HB-12317 NG108-15 小鼠神经细胞瘤与大鼠神经胶质瘤之融合细胞 的详细先容
HB-12317 NG108-15  小鼠神经细胞瘤与大鼠神经胶质瘤之融合细胞
ATCC® Number: HB-12317?    Price:
Designations: NG108-15 [108CC15]
Depositors:  Univ. Texas Southwestern Medical Cntr.
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Mus musculus (neuroblastoma); Rattus norvegicus (glioma) (mouse (neuroblastoma); rat (glioma))
Morphology: flat; round; 10 to 100 micrometers diameter
HB-12317 NG108-15 小鼠神经细胞瘤与大鼠神经胶质瘤之融合细胞
Source: Organ: brain
Disease: glioblastoma; neuroblastoma
Cell Type: somatic cell hybrid
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Applications: transfection host (Nucleofection technology from Lonza
Roche FuGENE® Transfection Reagents)
Comments: The NG108-15 cell line, originally named 108CC15, was developed in 1971 by Bernd Hamprecht. [112482]
The line was formed by fusing mouse N18TG2 neuroblastoma cells with rat C6-BU-1 glioma cells in the presence of inactivated Sendai virus. [51493]
Propagation: ATCC complete growth medium: The base medium for this cell line is Dulbeccos Modified Eagles Medium without sodium pyruvate . To make the complete growth medium, add the following components to the base medium:
  • 0.1 mM hypoxanthine (final conc.)
  • 400 nM aminopterin (final conc.)
  • 0.016 mM thymidine (final conc.)
  • 10% fetal bovine serum (final conc.)
    Temperature: 37.0°C
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:6 to 1:10 is recommended
Medium Renewal: Every 2 to 3 days
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Preservation: Freeze medium: Complete growth medium, 92.5%; DMSO, 7.5%
Storage temperature: liquid nitrogen vapor phase
Related Products: recommended serum:ATCC 30-2020
References: 51492: Hamprecht B. Structural, electrophysiological, biochemical, and pharmacological properties of neuroblastoma-glioma cell hybrids in cell culture. Int. Rev. Cytol. 49: 99-170, 1977. PubMed: 16829
51493: Hamprecht B, et al. Culture and characteristics of hormone-responsive neuroblastoma X glioma hybrid cells. Methods Enzymol. 109: 316-341, 1985. PubMed: 2985920
112482: Bernd Hamprecht, personal communication



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