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CL-101 LLC-PK1 猪肾细胞

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产品名称: CL-101 LLC-PK1 猪肾细胞
产品型号: CL-101
产品厂商: 美国标准生物品收藏中心(ATCC)
产品文档: 无相关文档


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CL-101 LLC-PK1 猪肾细胞 的详细先容
CL-101 LLC-PK1 猪肾细胞
ATCC® Number: CL-101™    Price:
Designations: LLC-PK1
Depositors:  Eli Lilly & Co.
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation
Organism:Sus scrofa (pig)

Source:Organ: kidney
Strain: Hampshire
Disease: normal
Cellular Products:plasminogen activator
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Age: 3 to 4 weeks
Gender: male
Propagation: ATCC complete growth medium: The base medium for this cell line is Medium 199 containing 1.5 g/L sodium bicarbonate. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 3%.
Temperature: 37.0°C
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended
Medium Renewal: Twice per week
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Preservation: culture medium 95%; DMSO, 5%
Related Products:recommended serum:ATCC 30-2020
formerly distributed as:ATCC CRL-1392
References:3520: Hull RN, Huseby RM. Enhanced production of plasminogen activator. US Patent 3,904,480 dated Sep 9 1975
22659: Perantoni A, Berman JJ. Properties of Wilms tumor line (TuWi) and pig kidney line (LLC-PK1) typical of normal kidney tubular epithelium. In Vitro 15: 446-454, 1979. PubMed: 225262
28301: Loffler S, et al. CD9, a tetraspan transmembrane protein, renders cells susceptible to canine distemper virus. J. Virol. 71: 42-49, 1997. PubMed: 8985321



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