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CRL-6323 B16F1 小鼠黑色素瘤细胞

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产品名称: CRL-6323 B16F1 小鼠黑色素瘤细胞
产品型号: CRL-6323
产品厂商: 美国标准生物品收藏中心(ATCC)
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CRL-6323 B16F1 小鼠黑色素瘤细胞,原代细胞|细胞系|细胞株|菌种;细胞库管理规范,提供的细胞株背景清楚,提供参考文献和*优培养条件!


CRL-6323 B16F1 小鼠黑色素瘤细胞 的详细先容
CRL-6323 B16F1 小鼠黑色素瘤细胞
ATCC® Number: CRL-6323™    Price: $275.00
Designations: B16-F1
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Mus musculus (mouse)
Morphology:
Source: Organ: skin
Strain: C57BL/6J
Disease: melanoma
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
 
Applications: transfection host (Roche FuGENE® Transfection Reagents)
Tumorigenic: Yes
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol: Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:10 is recommended
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: culture medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
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