bet亚洲官网手机版-bet1 365 vip官方网站


RF/6A 猴脉络膜-视网膜(内皮)细胞

如果您对该产品感兴趣的话,可以 sendmsg
产品名称: RF/6A 猴脉络膜-视网膜(内皮)细胞
产品型号: CRL-1780
产品厂商: 美国标准生物品收藏中心(ATCC)
产品文档: 无相关文档


CRL-1780 RF/6A 猴脉络膜-视网膜(内皮)细胞,原代细胞|细胞系|细胞株|菌种;细胞库管理规范,提供的细胞株背景清楚,提供参考文献和Z优培养条件!

RF/6A 猴脉络膜-视网膜(内皮)细胞 的详细先容
CRL-1780 RF/6A 猴脉络膜-视网膜(内皮)细胞
ATCC® Number: CRL-1780?    Price:
Designations: RF/6A
Depositors:  F Hu
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Macaca mulatta (monkey, rhesus)
Morphology: epithelial

Source: Tissue: choroid; retina
Disease: normal
Cell Type: endothelial
Cellular Products: factor VIII; fibronectin
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Age: fetus
Comments: This line spontaneously transformed at an early age and has been passaged over 540 times.
Weibel-Palade granules are seen by electron microscopy.
Late passage cells have been used as feeder layers to enhance attachment, subcultivation and maintenance of uveal melanocytes.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Subculturing: Protocol: Remove medium, add fresh 0.25% trypsin, 0.53 mM EDTA solution for 2 minutes at room temperature, remove trypsin and let the culture sit at 37C for 5 minutes (or until the cells detach). Add fresh medium, aspirate and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: Twice weekly
Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temperature
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003
recommended serum:ATCC 30-2020
References: 22315: Lou DA, Hu FN. Specific antigen and organelle expression of a long-term rhesus endothelial cell line. In Vitro Cell. Dev. Biol. 23: 75-85, 1987. PubMed: 3102454


细胞系,ATCC细胞,ATCC菌种,细胞系目录,ATCC,鼠伤寒沙门氏菌,atcc细胞库,人淋巴瘤细胞,人食管癌细胞,ATCC 19404,ATCC 25922,ATCC 19146,微生物菌种,细胞株,标准菌株,ATCC中国,atcc上海代理,CMCC 菌株,ATCC菌株,细胞目录,质控菌株,肿瘤细胞株,乳腺癌细胞系,人肝癌细胞系


Copyright? 2003-2021  上海复祥生物科技有限企业版权所有            电话:021-35080510
传真:021-35080520    地址:上海市虹口区赤峰路630号南雁大厦1901室    邮编:   


bet亚洲官网手机版|bet1 365 vip官方网站

XML 地图 | Sitemap 地图