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CCL-219 L1210 小鼠白血病细胞

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产品名称: CCL-219 L1210 小鼠白血病细胞
产品型号: CCL-219
产品厂商: 美国标准生物品收藏中心(ATCC)
产品文档: 无相关文档


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CCL-219 L1210 小鼠白血病细胞 的详细先容

CCL-219 L1210 小鼠白血病细胞
ATCC® Number:  CCL-219?     
Designations:  L1210 
Depositors:   EM Jensen 
Biosafety Level: 1 
Shipped:  frozen 
Medium & Serum:  See Propagation 
Growth Properties: suspension
Organism: Mus musculus (mouse) 
Morphology: lymphoblast

Source: Disease: lymphocytic leukemia
Strain: DBA subline 212
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. 
Tumorigenic: Yes 
Reverse Transcript: positive 
Cytogenetic Analysis: modal number = 40; range = 32 to 80.
Mouse karyotype with a diploid stemline number of 40 chromosomes.
Age:  8 months 
Gender:  female 
Comments: Tested and found negative for ectromelia virus (mousepox).
The line was established from a tumor in female #234 that developed following skin paintings with 0.2% methylcholanthrene in ether. [22513]
Propagation:  ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: horse serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing:  Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 5 X 10 exp4 viable cells/ml.
Medium Renewal: Add fresh medium (20% to 30% by volume) every 2 to 4 days
Preservation:  Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Doubling Time:  8 to 10 hrs 
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2040
References: 22513: . . J. Natl. Cancer Inst. 10: 179-192, 1949.
26055: . . J. Natl. Cancer Inst. 36: 405-421, 1966.
26056: . . Cancer Chemother. Rep. 51: 451-453, 1967.
32923: Young SW, et al. Gadolinium(III) texaphyrin: a tumor selective radiation sensitizer that is detectable by MRI. Proc. Natl. Acad. Sci. USA 93: 6610-6615, 1996. PubMed: 8692865
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